site-directed mutagenesis meaning in English
定点突变
定点诱变,定位诱变
Examples
- The principles , operation and applications of site - directed mutagenesis , gene fusion technology , and post - translational modification methods were introduced emphatically
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。 - We obtained the full length gene of hbfgf coding sequence with pcr , and adjusted the g + c content according the software dnasisv2 . 5 , and replaced the cys78 and cys96 with serines by site - directed mutagenesis . 2 . sequence result suggested one of the recombinant is correctly synthezied and cloned
用pcr方法合成了hbfgf编码区全长，其中前20个氨基酸的g + c含量按暨南大学硕士学位论文：大肠杆菌表达重组hbfgf结构和功能优化摘要照计算机软件计算的结果进行了调整，第78和96位上的半眯氨酸被突变为丝氨酸。